Device

Part:BBa_J44008:Design

Designed by: Adam Brown   Group: iGEM06_Missouri   (2006-08-22)


pLac-RBS-HinLVA-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1021
    Illegal NheI site found at 1357
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1081
    Illegal BamHI site found at 1503
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1529
    Illegal NgoMIV site found at 1897
    Illegal NgoMIV site found at 2057
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Possibility of read-thru of the pBADrev during transcription.


Source

(BBa_B0030), (BBa_B0015), (BBa_R0010), (BBa_J31007), (BBa_J3101)- from registry. (BBa_J31001) - from Davidson College. (BBa_J44000) - made in collaboration with Missouri Western/Davidson. (BBa_J44002) - from Missouri Western State University.

References